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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene is noticed in lots of tumors, which results in chromosome instability, and therefore may possibly contribute to tumorigenesis. This protein was uncovered to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a significant position in marketing mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are actually described. Two additional splice variants have been documented but in depth nucleotide sequence facts just isn't nonetheless obtainable.

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Blocking peptides are peptides that bind specially into the concentrate on antibody and block antibody binding. These peptide generally consists of the epitope acknowledged with the antibody.

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This guide outlines more attainable optimization of mobile and immunoassay parameters to be sure the very best success are...

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This tutorial outlines further more probable optimization of mobile and immunoassay parameters to ensure the very best results are...

Antibodies bound to the blocking peptide no longer bind to the epitope on the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is particular.

Target Information Cyclin E belongs into the really conserved cyclin spouse and children, whose customers are characterised by a spectacular periodicity in protein abundance throughout the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show distinctive expression and degradation designs which add on the temporal coordination of every mitotic event. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and is also degraded as cells development by way of S stage.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...

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